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Addgene

pcDNA4-FKBP-LAP1
(Plasmid #34841)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 34841 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA4
  • Backbone size w/o insert (bp) 5100
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FKBP-LAP1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    433
  • Promoter CMV
  • Tags / Fusion Proteins
    • myc (N terminal on insert)
    • T7 tag (N terminal on backbone)
    • His Tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV.F
  • 3′ sequencing primer pCDNA4.R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA4-FKBP-LAP1 was a gift from Alice Ting (Addgene plasmid # 34841 ; http://n2t.net/addgene:34841 ; RRID:Addgene_34841)

  • For your References section:

    Imaging Protein-Protein Interactions inside Living Cells via Interaction-Dependent Fluorophore Ligation. Slavoff SA, Liu DS, Cohen JD, Ting AY. J Am Chem Soc. 2011 Dec 14;133(49):19769-76. Epub 2011 Nov 18. 10.1021/ja206435e PubMed 22098454
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