pcDNA3-CD68 promoter/enhancer
(Plasmid #34837)

• Depositing Lab: Peter Murray
• Publication: Lang et al J Immunol. 2002 Apr 1;168(7):3402-11.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Modifications to backbone: CMV promoter has been removed
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CD68 promoter/enhancer -680/+140
• Species: H. sapiens (human)
• Insert Size (bp): 820
• Entrez Gene: CD68 (a.k.a. GP110, LAMP4, SCARD1)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: none (unknown if destroyed)
• 3′ cloning site: none (unknown if destroyed)
• 5′ sequencing primer: none
• 3′ sequencing primer: BGH_rev

Resource Information

• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The CD68 promoter/enhancer in this construct consists of ~680bp 5' of the ATG and the 83bp first intron of human CD68.

Please note that Addgene's sequencing results found a single nucleotide deletion at bp# 440 when compared to the full plasmid sequence provided by the depositor. The depositing scientist states that it is probably a polymorphism in the promoter and is not a concern for the function of the plasmid.