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Addgene

LZRS-GATA3
(Plasmid #34836)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 34836 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    LZRSpBMN-linker-IRES-EGFP
  • Backbone manufacturer
    Hergen Spits and coworkers
  • Backbone size w/o insert (bp) 12443
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GATA3
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1300
  • Entrez Gene
    Gata3 (a.k.a. Gata-3, jal)
  • Promoter LTR
  • Tags / Fusion Proteins
    • IRES (C terminal on backbone)
    • EGFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer EGFP-N
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LZRS-GATA3 was a gift from Ellen Rothenberg (Addgene plasmid # 34836 ; http://n2t.net/addgene:34836 ; RRID:Addgene_34836)

  • For your References section:

    GATA-3 expression is controlled by TCR signals and regulates CD4/CD8 differentiation. Hernandez-Hoyos G, Anderson MK, Wang C, Rothenberg EV, Alberola-Ila J. Immunity. 2003 Jul;19(1):83-94. 10.1016/S1074-7613(03)00176-6 PubMed 12871641
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