Flag-NRF1-HA
(Plasmid #34708)

• Depositing Lab: Raymond Deshaies
• Publication: Radhakrishnan et al Mol Cell. 2010 Apr 9;38(1):17-28.

Backbone

• Vector backbone: pMSCV-hyg
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 7000
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NRF1
• Alt name: nuclear factor erythroid-derived 2-related factor 1 (Nrf1)
• Alt name: NFE2L1
• Species: H. sapiens (human)
• Entrez Gene: NFE2L1 (a.k.a. LCR-F1, NRF-1, NRF1, TCF11)
• Promoter: LTR
• Tags / Fusion Proteins:
  • HA (C terminal on insert)
  • 3XFlag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (unknown if destroyed)
• 3′ cloning site: HpaI (unknown if destroyed)
• 5′ sequencing primer: pLXSN 5'

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry