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Addgene

pSox2-bd_mFGF_eGFP
(Plasmid #34704)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 34704 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    act-GVP-UG
  • Backbone manufacturer
    Created by Köster and Fraser
  • Vector type
    Xenopus expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    eGFP
  • Tags / Fusion Proteins
    • 6x Sox2/Oct3-4 transcription factor binding domain (N terminal on insert)
    • 165-bp minimal FGF4 promoter (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site none (unknown if destroyed)
  • 3′ cloning site none (unknown if destroyed)
  • 5′ sequencing primer pBluescriptSK_primer
  • 3′ sequencing primer EGFP_N
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This vector is the same as pSox2-bd::FP (Addgene #34703) except the Gal-UAS cassette (including the second mFGF) are not present. Please refer to plasmid 34703 for more information.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSox2-bd_mFGF_eGFP was a gift from Hollis Cline (Addgene plasmid # 34704 ; http://n2t.net/addgene:34704 ; RRID:Addgene_34704)

  • For your References section:

    In vivo time-lapse imaging of cell proliferation and differentiation in the optic tectum of Xenopus laevis tadpoles. Bestman JE, Lee-Osbourne J, Cline HT. J Comp Neurol. 2012 Feb 1;520(2):401-33. doi: 10.1002/cne.22795. 10.1002/cne.22795 PubMed 22113462
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