GST-PRMT7-ΔN
(Plasmid
#34694)
-
Depositing Lab
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 34694 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepGEX(SN)
-
Backbone manufacturerAmersham
- Backbone size w/o insert (bp) 5000
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namePRMT7
-
SpeciesH. sapiens (human)
-
Insert Size (bp)950
-
MutationContains aa 379–692
-
Entrez GenePRMT7 (a.k.a. SBIDDS)
- Promoter tac
-
Tag
/ Fusion Protein
- GST (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (unknown if destroyed)
- 3′ cloning site NotI (unknown if destroyed)
- 5′ sequencing primer pGEX 3' (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
GST-PRMT7-ΔN was a gift from Steven Clarke (Addgene plasmid # 34694 ; http://n2t.net/addgene:34694 ; RRID:Addgene_34694) -
For your References section:
PRMT7 is a member of the protein arginine methyltransferase family with a distinct substrate specificity. Miranda TB, Miranda M, Frankel A, Clarke S. J Biol Chem. 2004 May 28;279(22):22902-7. Epub 2004 Mar 24. 10.1074/jbc.M312904200 PubMed 15044439