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Addgene

GST-PRMT7
(Plasmid #34693)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 34693 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX(SN)
  • Backbone manufacturer
    Amersham
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PRMT7
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2100
  • Entrez Gene
    PRMT7 (a.k.a. SBIDDS)
  • Promoter tac
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer pGEX 3'
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GST-PRMT7 was a gift from Steven Clarke (Addgene plasmid # 34693 ; http://n2t.net/addgene:34693 ; RRID:Addgene_34693)

  • For your References section:

    PRMT7 is a member of the protein arginine methyltransferase family with a distinct substrate specificity. Miranda TB, Miranda M, Frankel A, Clarke S. J Biol Chem. 2004 May 28;279(22):22902-7. Epub 2004 Mar 24. 10.1074/jbc.M312904200 PubMed 15044439
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