G273D HA-dynamin 1 pUHD10-3
(Plasmid
#34689)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 34689 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUHD10-3
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namedynamin 1
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SpeciesH. sapiens (human)
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MutationG273D
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Entrez GeneDNM1 (a.k.a. DEE31, DNM, EIEE31)
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Tag
/ Fusion Protein
- HA (variant) (N terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
G273D HA-dynamin 1 pUHD10-3 was a gift from Sandra Schmid (Addgene plasmid # 34689 ; http://n2t.net/addgene:34689 ; RRID:Addgene_34689) -
For your References section:
Clathrin-independent pinocytosis is induced in cells overexpressing a temperature-sensitive mutant of dynamin. Damke H, Baba T, van der Bliek AM, Schmid SL. J Cell Biol. 1995 Oct;131(1):69-80. 10.1083/jcb.131.1.69 PubMed 7559787