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Addgene

K44A dynamin 1 pEGFP
(Plasmid #34681)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 34681 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pEGFP-N1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dynamin 1ba
  • Species
    H. sapiens (human)
  • Mutation
    K44A
  • Entrez Gene
    DNM1L (a.k.a. DLP1, DRP1, DVLP, DYMPLE, EMPF, EMPF1, HDYNIV, OPA5)
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    K44A dynamin 1 pEGFP was a gift from Sandra Schmid (Addgene plasmid # 34681 ; http://n2t.net/addgene:34681 ; RRID:Addgene_34681)
  • For your References section:

    An assembly-incompetent mutant establishes a requirement for dynamin self-assembly in clathrin-mediated endocytosis in vivo. Song BD, Yarar D, Schmid SL. Mol Biol Cell. 2004 May;15(5):2243-52. Epub 2004 Mar 5. 10.1091/mbc.e04-01-0015 PubMed 15004222