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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 34681 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-N1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namedynamin 1ba
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SpeciesH. sapiens (human)
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MutationK44A
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Entrez GeneDNM1L (a.k.a. DLP1, DRP1, DVLP, DYMPLE, EMPF, EMPF1, HDYNIV, OPA5)
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Tag
/ Fusion Protein
- EGFP (C terminal on insert)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
K44A dynamin 1 pEGFP was a gift from Sandra Schmid (Addgene plasmid # 34681 ; http://n2t.net/addgene:34681 ; RRID:Addgene_34681) -
For your References section:
An assembly-incompetent mutant establishes a requirement for dynamin self-assembly in clathrin-mediated endocytosis in vivo. Song BD, Yarar D, Schmid SL. Mol Biol Cell. 2004 May;15(5):2243-52. Epub 2004 Mar 5. 10.1091/mbc.e04-01-0015 PubMed 15004222