mg182
(Plasmid #34662)

• Depositing Lab: Miriam Goodman
• Publication: Johnson et al Proc Natl Acad Sci U S A. 2011 Nov 2.

Backbone

• Vector backbone: pBluescript KS(+)
• Backbone size w/o insert (bp): 2958
• Vector type: Xenopus oocyte expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Top 10 F' E. coli
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: slo-1(A2;B2;C1)
• Alt name: BK channel
• Alt name: Lablife ID:L1089.P202
• Species: C. elegans (nematode)
• Insert Size (bp): 3820
• GenBank ID: NM_001029090
• Entrez Gene: slo-1 (a.k.a. CELE_Y51A2D.19)
• Tags / Fusion Proteins:
  • Xenopus beta-globulin 5' UTR (N terminal on insert)
  • Xenopus beta-globulin 3' UTR (C terminal on insert)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The backbone plasmid containing insert slo-1(A2;B0;C1) was obtained from Zhao-Wen Wang (University of Connecticut)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The backbone plasmid containing insert slo-1(A2;B0;C1) was first published in: Wang, Z.W., Saifee, O., Nonet, M.L., and Salkoff, L. (2001). SLO-1 potassium channels control quantal content of neurotransmitter release at the C. elegans neuromuscular junction. Neuron 32, 867-881.