mg178
(Plasmid
#34658)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 34658 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBluescript KS(+)
- Backbone size w/o insert (bp) 2958
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Vector typeXenopus oocyte expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsTop 10 F' E. coli
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameslo-1(A1;B1;C1)
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Alt nameBK channel
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Alt nameLablife ID:L1089.P197
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SpeciesC. elegans (nematode)
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Insert Size (bp)3799
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GenBank IDNM_001029090
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Entrez Geneslo-1 (a.k.a. CELE_Y51A2D.19)
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Tags
/ Fusion Proteins
- Xenopus beta-globulin 5' UTR (N terminal on insert)
- Xenopus beta-globulin 3' UTR (C terminal on insert)
Resource Information
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A portion of this plasmid was derived from a plasmid made byThe backbone plasmid containing insert slo-1(A2;B0;C1) was obtained from Zhao-Wen Wang (University of Connecticut)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The backbone plasmid containing insert slo-1(A2;B0;C1) was first published in: Wang, Z.W., Saifee, O., Nonet, M.L., and Salkoff, L. (2001). SLO-1 potassium channels control quantal content of neurotransmitter release at the C. elegans neuromuscular junction. Neuron 32, 867-881.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mg178 was a gift from Miriam Goodman (Addgene plasmid # 34658 ; http://n2t.net/addgene:34658 ; RRID:Addgene_34658) -
For your References section:
Alternatively spliced domains interact to regulate BK potassium channel gating. Johnson BE, Glauser DA, Dan-Glauser ES, Halling DB, Aldrich RW, Goodman MB. Proc Natl Acad Sci U S A. 2011 Nov 2. 10.1073/pnas.1116795108 PubMed 22049343