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Addgene

GST-PRMT2
(Plasmid #34650)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 34650 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGex-6P-1
  • Backbone manufacturer
    Amersham
  • Backbone size w/o insert (bp) 4900
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PRMT2
  • Species
    H. sapiens (human)
  • Entrez Gene
    PRMT2 (a.k.a. HRMT1L1)
  • Promoter tac
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site None (unknown if destroyed)
  • 3′ cloning site None (unknown if destroyed)
  • 5′ sequencing primer pGEX 3'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GST-PRMT2 was a gift from Steven Clarke (Addgene plasmid # 34650 ; http://n2t.net/addgene:34650 ; RRID:Addgene_34650)

  • For your References section:

    Protein arginine methyltransferase 6 specifically methylates the nonhistone chromatin protein HMGA1a. Miranda TB, Webb KJ, Edberg DD, Reeves R, Clarke S. Biochem Biophys Res Commun. 2005 Oct 28;336(3):831-5. 10.1016/j.bbrc.2005.08.179 PubMed 16157300
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