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Addgene

pmTagBFP2-C1
(Plasmid #34634)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 34634 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pC1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4014
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mTagBFP2
  • Insert Size (bp)
    711
  • Mutation
    I174A compared to mTagBFP

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site BglII (not destroyed)
  • 5′ sequencing primer 5'-cggtaggcgtgtacggtgggag-3'
  • 3′ sequencing primer 5'-gttcagggggaggtgtgggagg-3'
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

There is an 11bp deletion in Addgene's sequencing results relative to the full plasmid sequence. This gap in alignment between nucleotides 595 and 606 results in a loss of AfeI and AgeI sites but does not influence expression of mTagBFP2 in mammalian cells.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pmTagBFP2-C1 was a gift from Vladislav Verkhusha (Addgene plasmid # 34634)

  • For your References section:

    An enhanced monomeric blue fluorescent protein with the high chemical stability of the chromophore. Subach OM, Cranfill PJ, Davidson MW, Verkhusha VV. PLoS One. 2011;6(12):e28674. Epub 2011 Dec 8. 10.1371/journal.pone.0028674 PubMed 22174863
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