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ptetmCherry_prey_plasmid-2
(Plasmid #34617)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 34617 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPROLar.A122
  • Backbone manufacturer
    Clonetech
  • Backbone size w/o insert (bp) 2070
  • Modifications to backbone
    ptet promoter before mCherry
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mCherry
  • Species
    Synthetic
  • Insert Size (bp)
    718
  • Promoter ptet

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AatII (not destroyed)
  • 3′ cloning site AvrII (not destroyed)
  • 5′ sequencing primer Unknown
  • 3′ sequencing primer Unknown
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Hao Song

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

MG1655 for expression

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ptetmCherry_prey_plasmid-2 was a gift from Lingchong You (Addgene plasmid # 34617 ; http://n2t.net/addgene:34617 ; RRID:Addgene_34617)

  • For your References section:

    Spatiotemporal modulation of biodiversity in a synthetic chemical-mediated ecosystem. Song H, Payne S, Gray M, You L. Nat Chem Biol. 2009 Dec;5(12):929-35. Epub 2009 Nov 1. 10.1038/nchembio.244 PubMed 19915540
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