8xGTIIC-luciferase
(Plasmid #34615)

• Purpose: YAP/TAZ-responsive synthetic promoter driving luciferase expression
• Depositing Lab: Stefano Piccolo
• Publication: Dupont et al Nature. 2011 Jun 8;474(7350):179-83. doi: 10.1038/nature10137.

Backbone

• Vector backbone: pGL3b
• Backbone size w/o insert (bp): 4800
• Vector type: Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: synthetic TEAD luciferase reporter
• Alt name: YAP/TAZ luciferase reporter
• Alt name: contains the minimal chicken TnT promoter
• Insert Size (bp): 360

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: NcoI (not destroyed)
• 5′ sequencing primer: RVprimer3
• 3′ sequencing primer: luciferase-rev

Resource Information

• Addgene Notes:
  • General advice and technical tips on how to monitor YAP/TAZ regulation and activity in cell culture by 8xG
• A portion of this plasmid was derived from a plasmid made by: The plasmid was derived from the 4XGTIIC-lux made from Ian Farrance, by adding 4 more TEAD binding sites (ACATTCCA).
• Articles Citing this Plasmid:
  • 189 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene sequencing detected a 7 nucleotide insertion at position 107 relative to the author's insert sequence. This results in the loss of a MscI restriction site but does not alter the function of the plasmid.

Please click http://www.addgene.org/34615/notes/ for general advice and technical tips on how to monitor YAP/TAZ regulation and activity in cell culture by 8xGTIIC lux.