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Addgene

LAMP1-mRFP-FLAG
(Plasmid #34611)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 34611 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLJM1
  • Backbone size w/o insert (bp) 7400
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    LAMP1
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1253
  • GenBank ID
    NM_012857.2
  • Entrez Gene
    Lamp1 (a.k.a. LGP120)
  • Tags / Fusion Proteins
    • mRFP (C terminal on insert)
    • 2xFlag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer pLJM1-3 (5'-GACGTGAAGAATGTGCGAGA-3')
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LAMP1-mRFP-FLAG was a gift from David Sabatini (Addgene plasmid # 34611 ; http://n2t.net/addgene:34611 ; RRID:Addgene_34611)

  • For your References section:

    mTORC1 senses lysosomal amino acids through an inside-out mechanism that requires the vacuolar H-ATPase. Zoncu R, Bar-Peled L, Efeyan A, Wang S, Sancak Y, Sabatini DM. Science. 2011 Nov 4;334(6056):678-83. 10.1126/science.1207056 PubMed 22053050
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