pLJM60
(Plasmid #34610)

• Depositing Lab: David Sabatini
• Publication: pLJM1-Flag (unpublished)

Backbone

• Vector backbone: pLJM1-Flag
• Backbone size w/o insert (bp): 7400
• Modifications to backbone: Made by cloning Age1- FLAG-Sal-gene-Not1-ExcoR1 into the Age1/Ecor1 sites in pLJM1
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: deptor
• Species: H. sapiens (human)
• Entrez Gene: DEPTOR (a.k.a. DEP.6, DEPDC6, hDEPTOR)
• Promoter: CMV
• Tag / Fusion Protein:
  • Flag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: need custom

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

If you plan on using this plasmid for the vector, know that it has a CMV promoter, a FLAG tag and then genes cloned into Sal/Not sites in the vector. Region of interest: Age(site destroyed)-FLAG-Sal-Gene-Not-EcoR1