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pBpuro_KLF4_ER
(Plasmid #34609)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 34609 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBABE-puro
  • Backbone manufacturer
    Morganstern JP and Land H 1990
  • Backbone size w/o insert (bp) 5169
  • Modifications to backbone
    none
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Growth instructions
    use amp 100ug/ml
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    KLF4
  • Alt name
    GKLF
  • Alt name
    EZF
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2400
  • GenBank ID
    AF105036
  • Entrez Gene
    KLF4 (a.k.a. EZF, GKLF)
  • Promoter LTR
  • Tags / Fusion Proteins
    • Myc epitope tag (N terminal on insert)
    • estrogen receptor fusion (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer use KLF4 internal primers
  • 3′ sequencing primer use KLF4 internal primers
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

To prepare vector, a BamHI fragment encoding c-MYC was released from pBABE-puro-cMYC-ER. See Littlewood TD, Hancock DC, Danielian PS, Parker MG and
Evan GI. (1995). Nucleic Acids Res., 23, 1686–1690. A BamHI fragment encoding KLF4 was inserted into the same sites of this vector.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBpuro_KLF4_ER was a gift from Michael Ruppert (Addgene plasmid # 34609 ; http://n2t.net/addgene:34609 ; RRID:Addgene_34609)

  • For your References section:

    Induction of KLF4 in basal keratinocytes blocks the proliferation-differentiation switch and initiates squamous epithelial dysplasia. Foster KW, Liu Z, Nail CD, Li X, Fitzgerald TJ, Bailey SK, Frost AR, Louro ID, Townes TM, Paterson AJ, Kudlow JE, Lobo-Ruppert SM, Ruppert JM. Oncogene. 2005 Feb 24;24(9):1491-500. 10.1038/sj.onc.1208307 PubMed 15674344
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