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Addgene

p413TEF-PKM2
(Plasmid #34608)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 34608 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    P413TEF
  • Backbone manufacturer
    ATCC#87362 (PMID:7737504)
  • Backbone size w/o insert (bp) 5557
  • Vector type
    Yeast Expression
  • Selectable markers
    HIS3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PKM2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1586
  • GenBank ID
    NM002654.4
  • Entrez Gene
    PKM (a.k.a. CTHBP, HEL-S-30, OIP3, PK3, PKM2, TCB, THBP1, p58)
  • Promoter TEF

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer pBluescriptSK
  • 3′ sequencing primer CYC1
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p413TEF-PKM2 was a gift from Markus Ralser (Addgene plasmid # 34608 ; http://n2t.net/addgene:34608 ; RRID:Addgene_34608)

  • For your References section:

    No evidence for a shift in pyruvate kinase PKM1 to PKM2 expression during tumorigenesis. Bluemlein K, Gruning NM, Feichtinger RG, Lehrach H, Kofler B, Ralser M. Oncotarget. 2011 May;2(5):393-400. 10.18632/oncotarget.278 PubMed 21789790
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