Skip to main content
Addgene

p413TEF-PYK2
(Plasmid #34606)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 34606 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    P413TEF
  • Backbone manufacturer
    ATCC#87362 (PMID:7737504)
  • Backbone size w/o insert (bp) 5557
  • Vector type
    Yeast Expression
  • Selectable markers
    HIS3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PYK2
  • Alt name
    YOR347C
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1521
  • Entrez Gene
    PYK2 (a.k.a. YOR347C)
  • Promoter TEF

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer pBluescriptSK
  • 3′ sequencing primer CYC1
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p413TEF-PYK2 was a gift from Markus Ralser (Addgene plasmid # 34606 ; http://n2t.net/addgene:34606 ; RRID:Addgene_34606)

  • For your References section:

    Pyruvate kinase triggers a metabolic feedback loop that controls redox metabolism in respiring cells. Gruning NM, Rinnerthaler M, Bluemlein K, Mulleder M, Wamelink MM, Lehrach H, Jakobs C, Breitenbach M, Ralser M. Cell Metab. 2011 Sep 7;14(3):415-27. 10.1016/j.cmet.2011.06.017 PubMed 21907146
Related items:
Commonly requested with: