P413TEF-PYK1
(Plasmid
#34605)
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 34605 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backboneP413TEF
-
Backbone manufacturerATCC#87362 (PMID:7737504)
- Backbone size w/o insert (bp) 5557
-
Vector typeYeast Expression
-
Selectable markersHIS3
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namePYK1
-
Alt nameYAL038W
-
SpeciesS. cerevisiae (budding yeast)
-
Insert Size (bp)1503
- Promoter TEF
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer pBluescriptSK
- 3′ sequencing primer CYC1 (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
P413TEF-PYK1 was a gift from Markus Ralser (Addgene plasmid # 34605 ; http://n2t.net/addgene:34605 ; RRID:Addgene_34605) -
For your References section:
Pyruvate kinase triggers a metabolic feedback loop that controls redox metabolism in respiring cells. Gruning NM, Rinnerthaler M, Bluemlein K, Mulleder M, Wamelink MM, Lehrach H, Jakobs C, Breitenbach M, Ralser M. Cell Metab. 2011 Sep 7;14(3):415-27. 10.1016/j.cmet.2011.06.017 PubMed 21907146