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Addgene

p416GPD-PYK1
(Plasmid #34604)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 34604 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p416GPD
  • Backbone manufacturer
    ATCC#87360 (PMID: 7737504
  • Backbone size w/o insert (bp) 5739
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PYK1
  • Alt name
    YAL038W
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1503
  • Promoter GPD

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer GPDPro-F
  • 3′ sequencing primer CYC1
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p416GPD-PYK1 was a gift from Markus Ralser (Addgene plasmid # 34604 ; http://n2t.net/addgene:34604 ; RRID:Addgene_34604)

  • For your References section:

    Pyruvate kinase triggers a metabolic feedback loop that controls redox metabolism in respiring cells. Gruning NM, Rinnerthaler M, Bluemlein K, Mulleder M, Wamelink MM, Lehrach H, Jakobs C, Breitenbach M, Ralser M. Cell Metab. 2011 Sep 7;14(3):415-27. 10.1016/j.cmet.2011.06.017 PubMed 21907146
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