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pMIR-Report-Luc-KLF4-K5S
(Plasmid #34598)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 34598 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMIR-Report-LUC
  • Backbone manufacturer
    Ambion
  • Backbone size w/o insert (bp) 6470
  • Vector type
    Mammalian Expression, Luciferase
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    KLF4
  • Alt name
    GKLF
  • Alt name
    EZF
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    306
  • Mutation
    Fragment 1781-2086 of KLF4
  • GenBank ID
    AF105036
  • Entrez Gene
    KLF4 (a.k.a. EZF, GKLF)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PmeI (destroyed during cloning)
  • 3′ cloning site PmeI (destroyed during cloning)
  • 5′ sequencing primer use KLF4 or firefly luciferase internal primers
  • 3′ sequencing primer use KLF4 or firefly luciferase internal primers
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This is a translation reporter. KLF4 cDNA (an 306 bp EcoRI fragment derived from the KLF4 3' UTR) was cloned into the PmeI site in the 3'UTR of the firefly luciferase transgene in pMIR-Report-Luciferase. The insert/cDNA fragment was generated by cleavage at KLF4-internal EcoRI sites within the KLF4 3' UTR, followed by Klenow treatment.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMIR-Report-Luc-KLF4-K5S was a gift from Michael Ruppert (Addgene plasmid # 34598 ; http://n2t.net/addgene:34598 ; RRID:Addgene_34598)

  • For your References section:

    A KLF4-miRNA-206 autoregulatory feedback loop can promote or inhibit protein translation depending upon cell context. Lin CC, Liu LZ, Addison JB, Wonderlin WF, Ivanov AV, Ruppert JM. Mol Cell Biol. 2011 Jun;31(12):2513-27. Epub 2011 Apr 25. 10.1128/MCB.01189-10 PubMed 21518959
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