pMIR-Report-Luc-KLF4-FL
(Plasmid #34597)

• Depositing Lab: Michael Ruppert
• Publication: Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27. Epub 2011 Apr 25.

Backbone

• Vector backbone: pMIR-Report-LUC
• Backbone manufacturer: Ambion
• Backbone size w/o insert (bp): 6470
• Vector type: Mammalian Expression, Luciferase
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: KLF4
• Alt name: GKLF
• Alt name: EZF
• Species: H. sapiens (human)
• Insert Size (bp): 2300
• GenBank ID: AF105036
• Entrez Gene: KLF4 (a.k.a. EZF, GKLF)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: PmeI (destroyed during cloning)
• 3′ cloning site: PmeI (destroyed during cloning)
• 5′ sequencing primer: use KLF4 internal primers
• 3′ sequencing primer: use KLF4 internal primers

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This is a translation reporter. KLF4 cDNA (the entire CDS and 3' UTR) was released from pCTV3K-SQC7 and cloned into the 3'UTR of the firefly luciferase transgene in pMIR-Report-Luciferase. The insert/cDNA fragment was generated by cleavage at a KLF4-internal Asp718 site within the KLF4 5' UTR, and a downstream SalI site. pCTV3K-SQC7 was described in Foster KW et al., Cell Growth & Differentiation, Vol. 10, 423–434, June 1999.
Please note that there is a G missing in the 3' UTR of KLF4.