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pBpuro - HA KLF4 FL delta K5S
(Plasmid #34592)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 34592 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBABE-puro
  • Backbone manufacturer
    Morganstern JP and Land H 1990
  • Backbone size w/o insert (bp) 5169
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    KLF4
  • Alt name
    GKLF
  • Alt name
    EZF
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2300
  • Mutation
    deletion of K5S, corresponding to bases 1781 to 2086 of GenBank accession number AF105036, by deletion of an internal EcoRI fragment; K5S contains the miR-206 and miR-344 binding sites.
  • GenBank ID
    AF105036
  • Entrez Gene
    KLF4 (a.k.a. EZF, GKLF)
  • Promoter LTR
  • Tag / Fusion Protein
    • HA tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (destroyed during cloning)
  • 5′ sequencing primer pBABE 5' see Addgene #1764
  • 3′ sequencing primer use KLF4 internal primers
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBpuro - HA KLF4 FL delta K5S was a gift from Michael Ruppert (Addgene plasmid # 34592 ; http://n2t.net/addgene:34592 ; RRID:Addgene_34592)

  • For your References section:

    A KLF4-miRNA-206 autoregulatory feedback loop can promote or inhibit protein translation depending upon cell context. Lin CC, Liu LZ, Addison JB, Wonderlin WF, Ivanov AV, Ruppert JM. Mol Cell Biol. 2011 Jun;31(12):2513-27. Epub 2011 Apr 25. 10.1128/MCB.01189-10 PubMed 21518959
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