pBpuro - HA KLF4 FL
(Plasmid #34589)

• Depositing Lab: Michael Ruppert
• Publication: Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27. Epub 2011 Apr 25.

Backbone

• Vector backbone: pBABE-puro
• Backbone manufacturer: Morganstern JP and Land H 1990
• Backbone size w/o insert (bp): 5169
• Modifications to backbone: none
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Growth instructions: use amp 100ug/ml
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: KLF4
• Alt name: GKLF
• Alt name: EZF
• Species: H. sapiens (human)
• Insert Size (bp): 2600
• GenBank ID: AF105036
• Entrez Gene: KLF4 (a.k.a. EZF, GKLF)
• Promoter: LTR
• Tag / Fusion Protein:
  • HA tag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (destroyed during cloning)
• 5′ sequencing primer: pBABE 5' see Addgene #1764
• 3′ sequencing primer: use KLF4 internal primers

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry