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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 34584 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonep3xFlag-CMV-7.1
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Backbone manufacturerSigma
- Backbone size w/o insert (bp) 4700
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSlug
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Alt nameSnai2
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SpeciesM. musculus (mouse)
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Insert Size (bp)800
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Entrez GeneSnai2 (a.k.a. Slug, Slugh, Snail2)
- Promoter CMV
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Tag
/ Fusion Protein
- 3xFLAG (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer CMV forward (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p3xFLAG-mSlug was a gift from Celeste Nelson (Addgene plasmid # 34584 ; http://n2t.net/addgene:34584 ; RRID:Addgene_34584) -
For your References section:
Snail1, Snail2, and E47 promote mammary epithelial branching morphogenesis. Lee K, Gjorevski N, Boghaert E, Radisky DC, Nelson CM. EMBO J. 2011 May 24;30(13):2662-74. doi: 10.1038/emboj.2011.159. 10.1038/emboj.2011.159 PubMed 21610693
Map uploaded by the depositor.
