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Purpose(Empty Backbone)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 34582 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonep3xFlag-CMV-7.1
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Backbone manufacturerSigma
- Backbone size (bp) 5400
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Modifications to backboneYFP open reading frame was amplified using specific primers and cloned into the p3xFlag-CMV-7.1 vector (Sigma) using BamHI and EcoRI restriction sites. Leaves HindIII, NotI, EagI and EcoRI sites between 3xHA and EYFP.
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Vector typeMammalian Expression
- Promoter CMV
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Tags
/ Fusion Proteins
- 3xFLAG (N terminal on backbone)
- EYFP (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p3xFLAG-EYFP was a gift from Celeste Nelson (Addgene plasmid # 34582 ; http://n2t.net/addgene:34582 ; RRID:Addgene_34582) -
For your References section:
Snail1, Snail2, and E47 promote mammary epithelial branching morphogenesis. Lee K, Gjorevski N, Boghaert E, Radisky DC, Nelson CM. EMBO J. 2011 May 24;30(13):2662-74. doi: 10.1038/emboj.2011.159. 10.1038/emboj.2011.159 PubMed 21610693
Map uploaded by the depositor.
