pcDNA3.1-3xHA-mASL
(Plasmid #34577)

• Purpose: NOTE: This plasmid contains 1xHA-mASL, not 3xHA-mASL.
• Depositing Lab: Peter Murray
• Publication: Peter Murray lab plasmids (unpublished)

Backbone

• Vector backbone: pcDNA3.1 hygro(+)
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5597
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ASL
• Species: M. musculus (mouse)
• Entrez Gene: Asl (a.k.a. 2510006M18Rik, ASAL)
• Promoter: CMV
• Tag / Fusion Protein:
  • 1xHA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: none (unknown if destroyed)
• 3′ cloning site: none (unknown if destroyed)
• 5′ sequencing primer: T7 forward

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry