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Addgene

WZLneo-PPARγ2
(Plasmid #34566)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 34566 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pWZL Neo
  • Backbone size w/o insert (bp) 6000
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PPARγ2
  • Alt name
    PPARg2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2100
  • Entrez Gene
    Pparg (a.k.a. Nr1c3, PPAR-gamma, PPAR-gamma2, PPARgamma, PPARgamma2)
  • Promoter 5'LTR
  • Tags / Fusion Proteins
    • Flag (N terminal on insert)
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer pWZL-Fwd
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    PPARγ2 obtained from B. Spiegelman (Addgene Plasmid #8859

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    WZLneo-PPARγ2 was a gift from Kai Ge (Addgene plasmid # 34566 ; http://n2t.net/addgene:34566 ; RRID:Addgene_34566)

  • For your References section:

    Histone H3K27 methyltransferase Ezh2 represses Wnt genes to facilitate adipogenesis. Wang L, Jin Q, Lee JE, Su IH, Ge K. Proc Natl Acad Sci U S A. 2010 Apr 20. 107(16):7317-22. 10.1073/pnas.1000031107 PubMed 20368440
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