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Addgene

pUC57-codon-modified HTK
(Plasmid #34563)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 34563 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC57
  • Backbone manufacturer
    Integrated DNA Technologies
  • Backbone size w/o insert (bp) 2728
  • Modifications to backbone
    Human Herpes Virus 1 (HSV-1)codon modified Thymidine Kinase gene was synthesized commercially and cloned into pUC57 by Integrated DNA Technologies.
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    codon-modified HTK
  • Species
    human herpes virus
  • Insert Size (bp)
    1311
  • Mutation
    codon modified HTK using mouse codon usage table
  • Promoter pUC57 bacteria promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer GTAAAACGACGGCCAGTG
  • 3′ sequencing primer GGAAACAGCTATGACCATG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC57-codon-modified HTK was a gift from David Anderson (Addgene plasmid # 34563 ; http://n2t.net/addgene:34563 ; RRID:Addgene_34563)

  • For your References section:

    A cre-dependent, anterograde transsynaptic viral tracer for mapping output pathways of genetically marked neurons. Lo L, Anderson DJ. Neuron. 2011 Dec 22;72(6):938-50. 10.1016/j.neuron.2011.12.002 PubMed 22196330
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