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Addgene

pWarf(+)
(Plasmid #34562)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 34562 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a(+)
  • Backbone manufacturer
    EMD
  • Backbone size w/o insert (bp) 5329
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HRV 3C-GPA-GFP-8HIS

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pWarf(+) was a gift from Jeff Abramson (Addgene plasmid # 34562 ; http://n2t.net/addgene:34562 ; RRID:Addgene_34562)

  • For your References section:

    Bridging the gap: a GFP-based strategy for overexpression and purification of membrane proteins with intra and extracellular C-termini. Hsieh JM, Besserer GM, Madej MG, Bui HQ, Kwon S, Abramson J. Protein Sci. 2010 Apr;19(4):868-80. 10.1002/pro.365 PubMed 20196076
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