pWarf(-)
(Plasmid
#34561)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 34561 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET28a(+)
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Backbone manufacturerEMD
- Backbone size w/o insert (bp) 5329
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)XL1 Blue
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHRV 3C-GFP-8His
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Insert Size (bp)758
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pWarf(-) was a gift from Jeff Abramson (Addgene plasmid # 34561 ; http://n2t.net/addgene:34561 ; RRID:Addgene_34561) -
For your References section:
Bridging the gap: a GFP-based strategy for overexpression and purification of membrane proteins with intra and extracellular C-termini. Hsieh JM, Besserer GM, Madej MG, Bui HQ, Kwon S, Abramson J. Protein Sci. 2010 Apr;19(4):868-80. 10.1002/pro.365 PubMed 20196076