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Addgene

pLyn-CaRQ
(Plasmid #34559)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 34559 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTriEx-3
  • Backbone manufacturer
    PMID: 12864922
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Mammalian Expression, Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pLyn-CaRQ
  • Alt name
    pLyn-CaM-MLCK- RhoA(Q63L)/IQp/RhoA
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1200
  • Mutation
    Q63L in RhoA
  • Entrez Gene
    RHOA (a.k.a. ARH12, ARHA, EDFAOB, RHO12, RHOH12)
  • Promoter CMV, T7
  • Tag / Fusion Protein
    • YFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV-F, T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

LYN membrane anchor - MGCIKSKGKDSATS
MLCK peptide - KRRWKKNFIAVSAANRFKKI
IQ peptide - GSAITVQRYVRGIQARAYARFL

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLyn-CaRQ was a gift from Kevin Truong (Addgene plasmid # 34559 ; http://n2t.net/addgene:34559 ; RRID:Addgene_34559)

  • For your References section:

    Ca2+-mediated synthetic biosystems offer protein design versatility, signal specificity, and pathway rewiring. Mills E, Truong K. Chem Biol. 2011 Dec 23;18(12):1611-9. 10.1016/j.chembiol.2011.09.014 PubMed 22195563
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