L57R
(Plasmid #34557)

• Depositing Lab: Kevin Truong
• Publication: Photo-activated Caspase-7 (unpublished)

Backbone

• Vector backbone: pTriEx-3
• Backbone manufacturer: PMID: 12864922
• Backbone size w/o insert (bp): 5000
• Vector type: Mammalian Expression, Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: L57
• Alt name: LOV2-Caspase-7 fusion
• Alt name: Caspase-7
• Alt name: Photoactivated Caspase-7
• Species: H. sapiens (human); A.Sativa (LOV2)
• Insert Size (bp): 1200
• Mutation: catalytic domain of caspase-7 was truncated at amino acid 57
• Entrez Gene: CASP7 (a.k.a. CASP-7, CMH-1, ICE-LAP3, LICE2, MCH3)
• Promoter: CMV, T7
• Tag / Fusion Protein:
  • mRFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (destroyed during cloning)
• 3′ cloning site: XhoI (destroyed during cloning)
• 5′ sequencing primer: CMV-F, T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

tandem fusion of LOV2 domain from A. Sativa phototropin 1, the catalytic domain of caspase-7 (human), and a mRFP variant that contains T140A and M141S mutations (introduced to facilitate cloning).