B23(NPM)
(Plasmid #34553)

• Depositing Lab: Mary Dasso
• Publication: Yun et al J Cell Biol. 2008 Nov 17;183(4):589-95.

Backbone

• Vector backbone: pCMV-DsRed-Express
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 4600
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: B23/nucleophosmin
• Species: H. sapiens (human)
• Insert Size (bp): 800
• Entrez Gene: NPM1 (a.k.a. B23, NPM)
• Promoter: CMV
• Tag / Fusion Protein:
  • DsRed (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (unknown if destroyed)
• 3′ cloning site: HindIII (unknown if destroyed)
• 5′ sequencing primer: CMV-Fwd
• 3′ sequencing primer: dsRed-N

Resource Information

• Articles Citing this Plasmid:
  • 7 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene quality control sequencing found an A123V point mutation, which, according to the depositing lab, should not alter the plasmid's function.