pTSK368
(Plasmid #34551)

• Depositing Lab: James McNally
• Publication: Karpova et al Science. 2008 Jan 25;319(5862):466-9.

Backbone

• Vector backbone: pTSK421
• Backbone manufacturer: T.S Karpova
• Modifications to backbone: See notes.
• Vector type: Yeast Expression
• Selectable markers: TRP1, HIS3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MS2-CP-NLS
• Promoter: Met25
• Tags / Fusion Proteins:
  • GFP (C terminal on insert)
  • GFP (C terminal on insert)
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: None (unknown if destroyed)
• 3′ cloning site: None (unknown if destroyed)
• 5′ sequencing primer: M13_puc_Rev
• 3′ sequencing primer: M13_puc_Fwd

Resource Information

• A portion of this plasmid was derived from a plasmid made by: MS2-CP-NLS obtained from R. Singer, Albert Einstein School of Medicine

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pTSK368 was constructed by modifying pTSK241 in the following way: pCap2 was replaced with pMet25 (ClaI-BamHI PCR fragment amplified
from genomic DNA) and Ace1p was replaced with MS2-CP-NLS (BamHI-NotI PCR fragment amplified from pMS2-YFP from R. Singer, Albert Einstein School of Medicine, Bronx, NY).