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Addgene

pTSK241
(Plasmid #34550)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 34550 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTSK65
  • Backbone manufacturer
    T.S Karpova
  • Vector type
    Yeast Expression ; Integrative Plasmid
  • Selectable markers
    TRP1, HIS3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ace1
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    CUP2 (a.k.a. YGL166W, ACE1)
  • Promoter CAP2
  • Tags / Fusion Proteins
    • GFP (C terminal on insert)
    • GFP (C terminal on insert)
    • GFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site None (unknown if destroyed)
  • 3′ cloning site None (unknown if destroyed)
  • 5′ sequencing primer M13_puc_Rev
  • 3′ sequencing primer M13_puc_Fwd
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTSK241 was a gift from James McNally (Addgene plasmid # 34550 ; http://n2t.net/addgene:34550 ; RRID:Addgene_34550)

  • For your References section:

    Concurrent fast and slow cycling of a transcriptional activator at an endogenous promoter. Karpova TS, Kim MJ, Spriet C, Nalley K, Stasevich TJ, Kherrouche Z, Heliot L, McNally JG. Science. 2008 Jan 25;319(5862):466-9. 10.1126/science.1150559 PubMed 18218898
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