Skip to main content
Addgene

pHYRSF1
(Plasmid #34549)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 34549 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pRSFDuet
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 3789
  • Modifications to backbone
    Second cloning site removed.
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DnaE N-intein
  • Alt name
    intein
  • Alt name
    NpuDnaE N-intein
  • Species
    Nostoc punctiforme
  • Insert Size (bp)
    313
  • Promoter T7
  • Tag / Fusion Protein
    • H6-GB1 (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7 fw
  • 3′ sequencing primer T7 rv
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHYRSF1 was a gift from Hideo Iwai (Addgene plasmid # 34549 ; http://n2t.net/addgene:34549 ; RRID:Addgene_34549)

  • For your References section:

    Segmental isotopic labeling of multi-domain and fusion proteins by protein trans-splicing in vivo and in vitro. Muona M, Aranko AS, Raulinaitis V, Iwaï H. Nat Protoc. 2010 . 5(3):574-87. 10.1038/nprot.2009.240 PubMed 20203672
Related items:
Commonly requested with: