KHBD00175
(Plasmid
#34328)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 34328 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepDONR221
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 2536
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Vector typeGateway Donor Vector
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCG5187
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Alt nameFBgn0035956
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Alt nameDoc2
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Alt nameDorsocross2
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SpeciesD. melanogaster (fly)
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Insert Size (bp)1407
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Entrez GeneDoc2 (a.k.a. Dmel_CG5187, CG5187, Dm-DOC2, Dmel\CG5187, Doc, doc2)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that the ORFs in this collection were cloned open-ended (without a stop codon).
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
KHBD00175 was a gift from Bart Deplancke (Addgene plasmid # 34328 ; http://n2t.net/addgene:34328 ; RRID:Addgene_34328) -
For your References section:
Automated protein-DNA interaction screening of Drosophila regulatory elements. Korneel Hens, Jean-Daniel Feuz, Alina Isakova, Antonina Lagovitina, Andreas Massouras, Julien Bryois, Patrick Callaerts, Susan E. Celniker, Bart Deplancke. Nat Methods. 2011 Oct 30;8(12):1065-70. 10.1038/nmeth.1763 PubMed 22037703