pIRES-Puro-TCPTP
(Plasmid
#33366)
-
Depositing Lab
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 33366 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepIRESpuro
-
Backbone manufacturerClontech
-
Vector typeMammalian Expression
-
Selectable markersPuromycin
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameTCPTP-45
-
SpeciesH. sapiens (human)
-
Entrez GenePTPN2 (a.k.a. PTN2, PTPT, TC-PTP, TC45, TC48, TCELLPTP, TCPTP)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site none (unknown if destroyed)
- 3′ cloning site none (unknown if destroyed)
- 5′ sequencing primer CMV forward (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pIRES-Puro-TCPTP was a gift from Ambra Pozzi (Addgene plasmid # 33366 ; http://n2t.net/addgene:33366 ; RRID:Addgene_33366) -
For your References section:
Integrin {alpha}1{beta}1 promotes caveolin-1 dephosphorylation by activating T cell protein-tyrosine phosphatase. Borza CM, Chen X, Mathew S, Mont S, Sanders CR, Zent R, Pozzi A. J Biol Chem. 2010 Dec 17;285(51):40114-24. Epub 2010 Oct 12. 10.1074/jbc.M110.156729 PubMed 20940300