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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 33365 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBG100
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Backbone manufacturerVanberbilt structural biology core plasmid
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Modifications to backbonepET27 derivative
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameTCPTP-45
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SpeciesH. sapiens (human)
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Entrez GenePTPN2 (a.k.a. PTN2, PTPT, TC-PTP, TC45, TC48, TCELLPTP, TCPTP)
- Promoter T7lac
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Tag
/ Fusion Protein
- 6xHIS - cleavable (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer T7 forward (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBG100-TCPTP was a gift from Ambra Pozzi (Addgene plasmid # 33365 ; http://n2t.net/addgene:33365 ; RRID:Addgene_33365) -
For your References section:
Integrin {alpha}1{beta}1 promotes caveolin-1 dephosphorylation by activating T cell protein-tyrosine phosphatase. Borza CM, Chen X, Mathew S, Mont S, Sanders CR, Zent R, Pozzi A. J Biol Chem. 2010 Dec 17;285(51):40114-24. Epub 2010 Oct 12. 10.1074/jbc.M110.156729 PubMed 20940300
Map uploaded by Addgene staff.