pIBG-FTet
(Plasmid #33361)

• Depositing Lab: Doug Mortlock
• Publication: Chandler et al Mol Cell Biol. 2007 Apr;27(8):2934-51. Epub 2007 Feb 5.

Backbone

• Vector backbone: pBluescript
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 3100
• Vector type: Mouse Targeting
• Selectable markers: Neomycin (select with G418) ; tetracycline

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B
• Growth instructions: Do not select for this plasmid with tetracycline, as we have found that the Tet resistance cassette does not work reliably in high-copy plasmids such as this one. The Tet cassette MUST be transferred to a low-copy vector, e.g. a BAC, to allow Tet resistance in a reliable fashion. In BAC clones it has been extremely reliable.
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: IRES-Bgeo
• Insert Size (bp): 5000

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: none (unknown if destroyed)
• 3′ cloning site: none (unknown if destroyed)
• 5′ sequencing primer: none
• 3′ sequencing primer: LacZ-R

Gene/Insert 2

• Gene/Insert name: TetR cassette
• Insert Size (bp): 2400
• Tags / Fusion Proteins:
  • FRT site (N terminal on insert)
  • FRT site (C terminal on insert)

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: none (unknown if destroyed)
• 3′ cloning site: none (unknown if destroyed)
• 5′ sequencing primer: Neo-F

Resource Information

• Supplemental Documents:
  • pIBGFtet_info_sheet
  • pIBGFtet_sequence

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Insert origin: IBG = Ires-Bgeo cassette from pGt1.8IresBgeo (Mountford et al (1994) PNAS 91:4303-4307); FRT-Tet-FRT cassette from pFRT129-NotX (Mortlock et al (2003) Genome Research 13:2069-2081).