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Addgene

CMV566 A-USF
(Plasmid #33360)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 33360 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    CMV566
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    USF
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    405
  • Mutation
    Dominant Negative (see notes below)
  • Entrez Gene
    Usf1 (a.k.a. bHLHb11)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site None (unknown if destroyed)
  • 3′ cloning site None (unknown if destroyed)
  • 5′ sequencing primer CMV-Fwd
  • 3′ sequencing primer BGH-Rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The A-USF aa sequence: MAYPYDVPDYAHMASMTGGQQMGRDPDEEEDDEEELEEDLENWIVQLSKIIPDCSMESTKSGQSKGGILSKACDYIQELRQSNHRLSEELQGLDQLQLDNDVLRQQVEDLKNKNLLLRAQLRHHGLEVVIKNDSN

This includes the N-terminal HA tag along with the acidic aa extension and the HLH-B-ZIP region of USF.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CMV566 A-USF was a gift from Charles Vinson (Addgene plasmid # 33360 ; http://n2t.net/addgene:33360 ; RRID:Addgene_33360)

  • For your References section:

    Cell-type-dependent activity of the ubiquitous transcription factor USF in cellular proliferation and transcriptional activation. Qyang Y, Luo X, Lu T, Ismail PM, Krylov D, Vinson C, Sawadogo M. Mol Cell Biol. 1999 Feb;19(2):1508-17. PubMed 9891084
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