pFRT-Tet 129
(Plasmid #33359)

• Depositing Lab: Doug Mortlock
• Publication: Mortlock lab unpublished plasmids (unpublished)

Backbone

• Vector backbone: pCRII
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 4100
• Vector type: Mouse Targeting

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): INValphaF'
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TetR cassette
• Insert Size (bp): 2400
• Tags / Fusion Proteins:
  • FRT site (N terminal on insert)
  • FRT site (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: none (unknown if destroyed)
• 3′ cloning site: none (unknown if destroyed)
• 5′ sequencing primer: M13 forward
• 3′ sequencing primer: M13 reverse

Resource Information

• Supplemental Documents:
  • pFRT-Tet129_info_sheet

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Do not select for this plasmid with tetracycline, as we have found that the Tet resistance cassette does not work reliably in high-copy plasmids such as this one. The Tet cassette MUST be transferred to a low-copy vector, e.g. a BAC, to allow Tet resistance in a reliable fashion. In BAC clones the Tet cassette has been extremely reliable.