CMV500 A-SREBP1
(Plasmid #33357)

• Depositing Lab: Charles Vinson
• Publication: Rishi et al J Biol Chem. 2004 Mar 19;279(12):11863-74. Epub 2003 Dec 31.

Backbone

• Vector backbone: CMV500
• Backbone size w/o insert (bp): 5500
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SREBP1
• Species: Cricetulus griseus (hamster)
• Insert Size (bp): 300
• Mutation: Dominant Negative (see notes)
• Entrez Gene: Srebf1 (a.k.a. SREBP-1, SREBP1)
• Promoter: CMV
• Tag / Fusion Protein:
  • FLAG (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: CMV-Fwd
• 3′ sequencing primer: BGH-Rev

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This is the dominant negative SREBP1- which contains an N-terminal acidic extension fused to the HLH-ZIP region of SREBP1.