pIRESNEO-Scambio-HA
(Plasmid
#33354)
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Depositing Labs
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 33354 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepIRESNEO
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 5300
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameScambio
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Alt nameSabi
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Alt nameARHGEF40
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SpeciesH. sapiens (human)
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Insert Size (bp)5200
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GenBank IDBAB84883 NM_018071
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Entrez GeneARHGEF40 (a.k.a. SOLO)
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Tag
/ Fusion Protein
- HA (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pIRESNEO-Scambio-HA was a gift from John Groffen & Nora Heisterkamp (Addgene plasmid # 33354 ; http://n2t.net/addgene:33354 ; RRID:Addgene_33354) -
For your References section:
Scambio, a novel guanine nucleotide exchange factor for Rho. Curtis C, Hemmeryckx B, Haataja L, Senadheera D, Groffen J, Heisterkamp N. Mol Cancer. 2004 Apr 23;3:10. 10.1186/1476-4598-3-10 PubMed 15107133