CMV566 empty vector
(Plasmid #33349)

• Purpose: (Empty Backbone)
• Depositing Lab: Charles Vinson
• Publication: Rishi et al J Biol Chem. 2004 Mar 19;279(12):11863-74. Epub 2003 Dec 31.

Backbone

• Vector backbone: pRc/CMV
• Backbone manufacturer: 5500
• Vector type: Mammalian Expression
• Promoter: CMV
• Tag / Fusion Protein:
  • HA (N terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The polylinker region of the pRc/CMV eukaryotic expression vector (Invitrogen) was cut with NdeI and HindIII, and the following annealed oligonucleotide was inserted with a hemagglutinin epitope (YPYDVPDYASL) and NdeI-HindIII cloning site was made using the following pair of complementary oligonucleotides: 5′-AGCTCCACCATGGCGTATCCCTACGACGTGCCCGATTATGCCCATATGTGATGAAGCTT-3′ (underlined bases are restriction sites for NcoI, NdeI, and HindIII) and 5′-CTAGAAGCTTCATCACATATGGGCATAATCGGGCACGTCGTAGGGATACGCCATGGTGG-3′ (underlined bases are restriction site for XbaI).