CMV500 empty vector
(Plasmid #33348)

• Purpose: (Empty Backbone)
• Depositing Lab: Charles Vinson
• Publication: Rishi et al J Biol Chem. 2004 Mar 19;279(12):11863-74. Epub 2003 Dec 31.

Backbone

• Vector backbone: Rc/CMV
• Backbone manufacturer: Invitrogen
• Backbone size (bp): 5500
• Vector type: Mammalian Expression
• Promoter: CMV
• Tag / Fusion Protein:
  • Flag (N terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The polylinker region of the pRc/CMV eukaryotic expression vector (Invitrogen) was cut with HindIII and XbaI, and the following annealed oligonucleotide was inserted: 5′-AGCTCCACCATGGACTACAAGGACGACGATGACAAGCATATGTGATGAAGCTT-3′ (underlined bases are restriction sites for NcoI, NdeI, and HindIII) and 5′-CTAGAAGCTTCATCACATATGCTTGTCATCGTCGTCCTTGTAGTCCATGGTGG-3′ (underlined bases are restriction site for XbaI). The HindIII restriction site was not regenerated and was reintroduced downstream of the FLAG sequence (DYKDDDDK).