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Addgene

pLEW100v5-trunc
(Plasmid #33346)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 33346 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEM
  • Backbone size w/o insert (bp) 4417
  • Vector type
    Trypanosoma brucei expression vector
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Luciferase truncated to allow cytoplasmic instead of peroxisomal (glycosomal) expression
  • Species
    Firefly
  • Insert Size (bp)
    1632
  • Mutation
    21bp of wt luciferase deleted

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer LucNrev
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid is a derivative of Addgene plasmid 24011: pLEW100v5.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLEW100v5-trunc was a gift from George Cross (Addgene plasmid # 33346 ; http://n2t.net/addgene:33346 ; RRID:Addgene_33346)
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